Journal: Frontiers in oncology

Article Title: Proteomic Characterization of Cytoplasmic Lipid Droplets in Human Metastatic Breast Cancer Cells.

doi: 10.3389/fonc.2021.576326

Figure Lengend Snippet: FIGURE 7 | SQLE and NSDHL localize to cytoplasmic lipid droplets (CLDs) in MCF10CA1a cells. Representative immunofluorescence images of MCF10CA1a cells. Cells were stained with Alexa Fluor 633 to visualize SQLE (A) and NSDHL (B), BODIPY to visualize CLDs, and DAPI to visualize nuclei. Signals from all three channels were merged for the final image in (A, B).

Article Snippet: Cells were probed with antibodies for PLIN3, SQLE, and NSDHL (Sigma, HPA006427; SantaCruz Biotechnologies, sc-271651; Atlas Antibodies, HPA000571, respectively).

Techniques: Staining

Journal: Cell reports

Article Title: ATGL/CGI-58-Dependent Hydrolysis of a Lipid Storage Pool in Murine Enterocytes

doi: 10.1016/j.celrep.2019.07.030

Figure Lengend Snippet: (A) Radioactivity in the SI and liver of chow diet-fed WT and iDKO mice (n = 4 or 5) 30 min post-gavage of 2 μCi [9,10- 3 H(N)]-triolein in 200 μL corn oil. (B) ORO staining of duodenal sections 30 min after an oral lipid load. (C and D) Biochemical (C) and histological (D) analysis of hepatic lipid levels 30 min post-gavage of 200 μL corn oil. (E and F) Lipid concentrations (E) and (F) lipoprotein profiles in the plasma 30 min post-gavage of corn oil (200 μL). Data represent mean + SD (n = 3 or 4). *p < 0.05, **p ≤ 0.01, and ***p ≤ 0.001. Magnification, 40×; scale bar, 50 μm. (G and H) Chow diet-fed mice were fasted for 16 h prior to an oral administration of 100 μL corn oil containing 1 μg/g body weight BODIPY FL C 16 (green). Intestinal sections were co-stained with PLIN3 (red) to visualize colocalization with cLDs. PLIN3 immunofluorescence staining 30 min (G) and 2 h (H) post-gavage. Green background fluorescence results from high chlorophyll content in the chow diet (alfalfa). Arrows indicate cLDs originating from BODIPY-labeled FA, which colocalize with PLIN3; arrowheads indicate endogenous cLDs coated with PLIN3; stars indicate BODIPY-containing cLDs, which do not colocalize with PLIN3. Magnification, 100×; scale bar, 10 μm. CE, cholesteryl esters; Duo, duodenum; FC, free cholesterol; HDL, high-density lipoprotein; Hep, hepar (liver); Ile, ileum; Jej, jejunum; LDL, low-density lipoprotein; PLIN3, Perilipin 3; Sto, stomach; TC, total cholesterol; TG, triglycerides; VLDL, very low density lipoprotein. See also .

Article Snippet: Sections were incubated overnight at 4°C with the primary antibody against PLIN3 (abs482; 1:200; Merck KGaA, Darmstadt, Germany), followed by incubation with the corresponding Alexa Fluor® 594-labeled secondary antibody for PLIN3 (A11037; 1:250; Thermo Fisher Scientific, Waltham, MA).

Techniques: Radioactivity, Staining, Clinical Proteomics, Immunofluorescence, Fluorescence, Labeling

Journal: Cell reports

Article Title: ATGL/CGI-58-Dependent Hydrolysis of a Lipid Storage Pool in Murine Enterocytes

doi: 10.1016/j.celrep.2019.07.030

Figure Lengend Snippet:

Article Snippet: Sections were incubated overnight at 4°C with the primary antibody against PLIN3 (abs482; 1:200; Merck KGaA, Darmstadt, Germany), followed by incubation with the corresponding Alexa Fluor® 594-labeled secondary antibody for PLIN3 (A11037; 1:250; Thermo Fisher Scientific, Waltham, MA).

Techniques: Diagnostic Assay, Software, Imaging